Figure 1.
![Effect of high extracellular [K+] on collagen-plus-thrombin–induced platelet procoagulant response. Platelets at 107 mL–1 were activated with collagen (10 μg/mL) plus thrombin (4 nM) for 10 minutes in HEPES, HEPES/choline, or HEPES/KCl, and the procoagulant response was measured as the binding of FITC-conjugated annexin A5. Data are expressed as a percentage of the response obtained in HEPES. We emphasize that the 100% value in HEPES corresponds to approximately 20% to 25% of the total cell population that becomes positive for annexin binding, depending on the donor. Data are mean values ± 1SD(n = 4).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/108/7/10.1182_blood-2006-01-009613/4/zh80190601770001.jpeg?Expires=1722255019&Signature=2AasTexBpJZpYFlEhIjQV9R~Y5zpEAD7Xn99-QyVX4LKZafLu70G7rLDrfgQJ~q-pVbUJl5ka~Xqx8A00ywgetmPyNMjUvhrEN3ygGJWGHJs-bjOnewKrXKB~APpQ9dWI9P5F2tnMaJC1rrzLg3DLWKYJUkf561rB3-OTuT8OTAqw3d5KCWQPvMeMtJ4Z1AWJEDZjcEwXi8z7HRjq-wg1QtGupwRY7lbwdvOHQmMuDwG58USc0HiB~xKee3NB4OTwzGQcV3MgEMNaReJ5l0Fx3UY~pmIsgIfbPiNr8G23~Oa5EIwq~3WsPnxRKjq6aEsCcmLYtranCvLo7qwXP~l8w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of high extracellular [K+] on collagen-plus-thrombin–induced platelet procoagulant response. Platelets at 107 mL–1 were activated with collagen (10 μg/mL) plus thrombin (4 nM) for 10 minutes in HEPES, HEPES/choline, or HEPES/KCl, and the procoagulant response was measured as the binding of FITC-conjugated annexin A5. Data are expressed as a percentage of the response obtained in HEPES. We emphasize that the 100% value in HEPES corresponds to approximately 20% to 25% of the total cell population that becomes positive for annexin binding, depending on the donor. Data are mean values ± 1SD(n = 4).
