Ang2 antagonized Ang1 stimulation of endothelial cell–induced SMC migration. HUVECs were infected with a control vector, AdAng1, and AdAng1 plus AdAng2 for 48 hours. SMC migration was evaluated with the EC-SMC coculture assay. Migrated SMCs were counted in randomly selected fields. Experiments were repeated at least 3 times in more than 2 wells for each treatment. Five fields were counted for each filter in each experiment. *P < .01 compared with control. **P < .01 compared with Ang1 group.