Ang1 induced HGF expression in HUVECs. (A) Total RNAs were extracted from corresponding adenoviral vector–infected HUVECs for 48 hours. HGF mRNA was analyzed by Northern blot. 18S rRNA, visualized with ethidium bromide and UV, was used as a loading control. Images are representative of 3 separate experiments. (B) Cells were lysed from corresponding adenoviral vector–infected HUVECs for 48 hours. Cell lysates were analyzed by Western blotting and probed with an anti-HGF antibody. (C) Cell-conditioned media were collected, concentrated, and analyzed for protein expression by Western blotting. Filters were immunoblotted with anti-Ang1–, anti-Ang2–, and anti-VEGF–specific antibodies, respectively.