PTX3 activity is IL-12/IFN-γ dependent and TLR9/MyD88 independent. Viral load and cytokine production in different types of mice upon MCMV infection and PTX3 treatment. Animals were infected intraperitoneally with 105 (BALB/c, IFN-γ–/–) or 5 × 105 (C57BL6, IL-12p40–/–, IFN-αβ–/–, TLR2–/–, TLR3–/–, TLR4–/–, TLR9–/–, and MyD88–/–) PFU of MCMV. Virus titers, expressed as log10, were quantified on MEF cells by standard plaque assay on lung tissues removed at 7 days after infection. PTX3 (1 mg/kg intraperitoneally) was administered daily beginning on the day of the infection and continuing for 1 week. Controls received the diluent alone. Cytokine (pg/mL) levels in culture supernatants of spleen cells (day 7) were determined by ELISA assay. Bars indicate the standard errors. Results are representative of 3 independent experiments. *P < .05, PTX3-treated versus untreated mice.
