Detection of BCL6 translocations in gastric lymphoma. (A) Ethidium-bromide–stained gel showing BCL6 translocation products detected by various methods in all cases with non-Ig and examples of Ig as BCL6 fusion partner (5′-RACE on mRNA, inverse PCR on genomic DNA, and long-range PCR on genomic DNA). The case number is shown above each lane. cDNA indicates purified control cDNA amplified with control forward and reverse primers; dA, poly dA-tailed control cDNA amplified with oligo-dT anchor primer and the control reverse primer. (B) Top panel shows restriction map of the BCL6 gene, and distribution of the different breakpoints of BCL6 translocations identified in this study. Small vertical lines below the horizontal line indicate the positions of different translocation breakpoints. MTC indicates the major translocation breakpoints cluster; MMC, the major mutation cluster. Restriction sites: E, EcoRI; B, BamHI; H, HindIII; X, XbaI; S, SacI; Xh, Xhol. Lower panel shows the magnified description of top panel showing the region with different translocation breakpoints. The fusion partners of each BCL6 translocation are indicated. *DLCLML cases.