Induction of FOXP3⁺ T cells in CD4⁺CD25^- T cells by DCs. (A) CD25^- T cells were isolated by flow sorting and cultured alone (T alone) or with Cyt-DCs. After 7 days of culture, CD4⁺CD25⁺ FOXP3⁺ T cells were monitored by flow cytometry. One of 4 similar experiments. (B) CD25^- T cells were cultured alone or with Cyt-DCs, and expression of FOXP3 protein was monitored by flow cytometry on days 0, 1, 4, and 7 of culture. The graph shows a summary of 2 experiments (mean ± SD).