Apoptosis following EGCG treatment of myeloma cells. Myeloma cells were treated with 10 μM EGCG for 72 hours and analyzed for apoptosis using annexin V-biotin apoptosis detection kit. Cells were sequentially treated with annexin V-biotin and FITC-streptavidin. FITC-streptavidin-labeled apoptotic cells within the same microscopic field were viewed and photographed by phase contrast (PC) or by fluorescence emitted at 518 nm (FITC filter). Using the FITC filter, apoptotic cells appear bright green. (A) INA6 myeloma cells, untreated (i-ii) or treated with EGCG (iii-iv). (B) Bar graph shows percent apoptotic cells in control or EGCG-treated INA6 cells. (C) ARP myeloma cells, untreated (i-ii) or treated with EGCG (iii-iv). (D) Bar graph shows percent apoptotic cells in control or EGCG-treated ARP cells. (B, D) Error bars indicate SEM of percentage of apoptotic cells in 5 distinct microscopic fields.