American Society of Hematology

Figure 6.

$Figure 6. PTPROt overexpression inhibits SHC and BLNK tyrosyl phosphorylation. Tet-inducible FLAG-tagged WT or mutant (DA and CS) PTPROt clones were cultured with or without Dox, serum starved, and stimulated with goat anti–human IgG for 8 minutes or left untreated. Thereafter, cells were lysed and immunoprecipitated with anti-SHC or anti-BLNK antibody. SHC immunoprecipitates were size fractionated, blotted, and analyzed with the phosphotyrosine antibody 4G10 (top panel). The blot was subsequently stripped and blotted with an anti-SHC antibody (bottom panel). BLNK immunoprecipitates were size fractionated, blotted, and analyzed with the phosphotyrosine antibody 4G10 (top panel). The blot was subsequently stripped and blotted with an anti-BLNK antibody (bottom panel).$

PTPROt overexpression inhibits SHC and BLNK tyrosyl phosphorylation. Tet-inducible FLAG-tagged WT or mutant (DA and CS) PTPROt clones were cultured with or without Dox, serum starved, and stimulated with goat anti–human IgG for 8 minutes or left untreated. Thereafter, cells were lysed and immunoprecipitated with anti-SHC or anti-BLNK antibody. SHC immunoprecipitates were size fractionated, blotted, and analyzed with the phosphotyrosine antibody 4G10 (top panel). The blot was subsequently stripped and blotted with an anti-SHC antibody (bottom panel). BLNK immunoprecipitates were size fractionated, blotted, and analyzed with the phosphotyrosine antibody 4G10 (top panel). The blot was subsequently stripped and blotted with an anti-BLNK antibody (bottom panel).

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