Figure 2.
Microdissection and mutational analysis of a representative patient with NLPHL. (A) Lymph node biopsy showing a neoplastic nodule containing characteristic L/H cells with popcorn appearance of the nucleus (top left, hematoxylineosin stain; magnification, 800 ×). (Top middle, immunoperoxidase technique; magnification, ×150. L/H tumor cells are CD20+ (arrows) and are surrounded by a rim of CD20– rosette T cells (top right, immunoperoxidase technique; magnification, 800 ×). CD20+ L/H cells before and after laser microdissection (bottom, right and left) (APAAP technique). *CD20+ tumor nodule. (B) Electropherogram showing mutations of the RhoH/TTF and PAX5 genes in microdissected L/H cells (Table 2, patient 2).

Microdissection and mutational analysis of a representative patient with NLPHL. (A) Lymph node biopsy showing a neoplastic nodule containing characteristic L/H cells with popcorn appearance of the nucleus (top left, hematoxylineosin stain; magnification, 800 ×). (Top middle, immunoperoxidase technique; magnification, ×150. L/H tumor cells are CD20+ (arrows) and are surrounded by a rim of CD20 rosette T cells (top right, immunoperoxidase technique; magnification, 800 ×). CD20+ L/H cells before and after laser microdissection (bottom, right and left) (APAAP technique). *CD20+ tumor nodule. (B) Electropherogram showing mutations of the RhoH/TTF and PAX5 genes in microdissected L/H cells (Table 2, patient 2).

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