HUVECs and HMVECs express the CD160 receptor. (A) HUVECs, HMVECs, HAOSMCs, and fibroblasts in primary culture were analyzed by flow cytometry after incubation with anti-CD8-, anti-CD85d-, anti-CD85j-, or CL1-R2 (anti-CD160)-specific mAbs (open profiles) or IgG isotype controls (filled profiles), followed by PE-labeled conjugates. Results are representative of 6 independent experiments. (B) CD160 mRNA was expressed by HUVECs and NK92 cells (positive control) but notbyCD4⁺ T cells (negative control). RT-PCR analysis, using CD160-specific primers, compared with β-actin control primers. (C) Predicted amino acid sequence alignment of CD160 expressed in HUVECs and NK92. Dotted lines indicate identity.