WNT5A expression in human monocyte-derived and alveolar macrophages. Qualitative detection of WNT5A mRNA expression by in situ hybridization with a WNT5A-specific probe (top row). Human monocyte-derived macrophages (hMDM) were analyzed after cultivation for 4 hours. Human uninvolved lung tissues obtained during tumor surgery (5 individuals; one representative shown) were analyzed. (Bottom row) In situ hybridization with a nonspecific scrambled DNA probe.