Comparison of CD16 modulation, CD54 activation, and ADCC. The EC₅₀ for AME-D and R was evaluated for CD16 modulation (A), CD54 up-regulation (B), and ADCC (C) and plotted on a log scale and grouped by polymorphism (n = 4 VV, 4 VF, and 4 FF for each assay). Similar results were found whether the readout was loss of surface CD16, NK activation as determined by CD54 up-regulation, or lysis of target cells as determined by ADCC. Error bars indicate SEM.