Steap mRNA expression by in situ hybridization. E15.5 mouse embryo sections were analyzed for Steap mRNA expression by 35S-radioactive in situ hybridization. (A) Bright field image; (B) inverted dark field negative control probe (Steap1 sense RNA); (C) Steap1 (antisense RNA), note adrenal staining (▸); (D) Steap2 (antisense RNA); (E) Steap2 higher magnification of the liver (▾) and gastroduodenal junction (▸); (F) Steap4. Checkerboard background is the result of assembling the low-power image from multiple high-power images using Compix software (see “Materials and methods”).