IL-2 promoter activation by BCL11B requires the region between -243 and -190. (A) Schematic representation of the IL-2 promoter deletion mutants. (B) Luciferase reporter assays from MSCV-BCL11B (▪) and MSCV (□) Jurkat cells transfected with the IL-2 promoter deletion mutants. Ratios of luciferase activity between MSCV-BCL11B and MSCV Jurkat cells for each deletion mutant are shown. Reporter assays were conducted after treatment of the cells with PMA/ionomycin for 8 hours. The quantifications represent means of 3 independent experiments ± SD.