Effects of C/EBPα on 32D-BCR/ABL cells numbers. (A) Schematic diagram of wild-type and mutant C/EBPα-ER^TAM chimeric proteins. (B) Levels of endogenous C/EBPα and ectopic C/EBPα-ER^TAM in 32Dcl3 (lanes 1-2), in 32D-BCR/ABL cells (lanes 3-4), or in 32D-BCR/ABL cells transduced with the MigRI empty vector (lane 5) or wild-type or mutant C/EBPα-ER^TAM (lanes 6-8). The anti-C/EBPα blot was exposed for 2 minutes (lanes 1-4) or 10 seconds (lanes 5-8). (C) Cell counts of 4-HT–treated MigRI- or C/EBPα-ER^TAM–transduced 32D-BCR/ABL cells. Values represent mean ± SD of 3 different experiments.