Figure 1.
SDF-1 production in cocultures. Cocultures with BC cell lines (T47D, ZR-75-30, BT383) and primary BCCs (P1, P2, P4). Each BCC was studied with stroma from a different donor. At 80% confluence, BCCs were positively selected and each (106/mL) replated separately in stromal media. After 24 hours, the culture media were analyzed by protein microarrays and by ELISA for SDF-1α. The densities of internal positive controls were arbitrarily assigned values of 10 and then used as the base for unknowns. The ELISA values, in mean picograms per milliliter ± SD (n = 6), are placed above the respective bars. *P < .05 versus individual BCC culture.
