NRP2 knock-down inhibits HMVEC migration induced by VEGF-A and VEGF-C. (A) Migration assay of HMVECs transfected with control or NRP-2 siRNA. After 36 hours, transfected cells were seeded in triplicate in the upper wells of a fluoroblok chamber. RPMI alone or containing VEGF-A 50 ng/mL or VEGF-C 100 ng/mL was added in the bottom wells. The amount of migrating cells was quantified using calcein labeling. Data are shown as mean ± SEM. (B) HMVEC migration induced by VEGF-C 100 ng/mL in the presence of increasing doses (1-100 nM) of CEP5214 kinase inhibitor. Results are representative of 3 independent experiments.