Regulation of the glucocorticoid receptor upon prednisolone exposure in ALL. Leukemic cells were incubated in culture medium with or without 250 μg/mL prednisolone for 3, 8, and 24 hours. The total GR mRNA expression as the sum of GR-alpha, GR-beta, and GR-P for both the prednisolone-incubated and control samples is depicted. The results are depicted as median (horizontal bars) and 25th and 75th percentiles.