Figure 4.
Figure 4. Hypersensitivity of Fancc–/– cells to IFNγ/TNFα or mitomycin C can be suppressed by expression of the dominant negative RAX(S18A) mutant. (A) Western blots with lysates from Fancc–/– MEF cells expressing either RAX or RAX(S18A). (B) Survival as determined using TUNEL assay after treatment with 10 ng/mL IFNγ and/or 10 ng/mL TNFα of Fancc–/– cell lines. (C) Autoradiographs and Western blots from 3 separate PKR autophosphorylation assays were quantified and used to graph the increase in active PKR relative to total PKR protein level after treatment with 10 ng/mL IFNγ for 24 hours followed by treatment with 10 ng/mL TNFα for the indicated times. (D) Survival of normal MEF and Fancc–/– MEF cell lines expressing either exogenous RAX or RAX(S18A) after 24 hours of treatment with the indicated concentrations of mitomycin C. Viability was measured using TUNEL assay.

Hypersensitivity ofFancc–/–cells to IFNγ/TNFα or mitomycin C can be suppressed by expression of the dominant negative RAX(S18A) mutant. (A) Western blots with lysates from Fancc–/– MEF cells expressing either RAX or RAX(S18A). (B) Survival as determined using TUNEL assay after treatment with 10 ng/mL IFNγ and/or 10 ng/mL TNFα of Fancc–/– cell lines. (C) Autoradiographs and Western blots from 3 separate PKR autophosphorylation assays were quantified and used to graph the increase in active PKR relative to total PKR protein level after treatment with 10 ng/mL IFNγ for 24 hours followed by treatment with 10 ng/mL TNFα for the indicated times. (D) Survival of normal MEF and Fancc–/– MEF cell lines expressing either exogenous RAX or RAX(S18A) after 24 hours of treatment with the indicated concentrations of mitomycin C. Viability was measured using TUNEL assay.

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