Kinetics of STAT3-C–transduced CRU expansion in mice that underwent transplantation. (A) The numbers of GFP⁺ CRUs present in primary recipients of 4 × 10⁵ transduced bone marrow cells from 5-FU–pretreated Pep3b mice was assessed at the indicated time points. Transduced bone marrow cells were transplanted into primary mice (9 mice per group), and then at each time point indicated, bone marrow cells from 3 primary mice in each group were pooled and transplanted into secondary recipients to determine the number of transduced (GFP⁺) CRU numbers present. Values shown are the mean ± 2 SEM (95% CI). The number of transduced CRUs initially injected was determined in a separate limiting-dilution experiment. (B) Number of GFP⁺ CRUs regenerated over time in 3 individually assessed primary recipients of single STAT3-C–transduced CRUs (SC nos. 1-3, Figure 2B). Values shown are the mean ± 2 SEM.