Figure 3.
![Figure 3. Genomic PCR-SSCP analysis of RFC exon 3 in parental cells and their PT523-resistant sublines. High-molecular-weight gDNA (∼50 ng) isolated from parental CCRF-CEM cells and their various antifolate-resistant sublines was PCR amplified in the presence of [32P]dCTP using oligonucleotide primers targeting exon 3. The PCR products were resolved by electrophoresis on nondenaturing, 8% polyacrylamide gels, as described in “Materials and methods.” The arrowhead shown on the right side depicts the band with the altered electrophoretic mobility.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/8/10.1182_blood-2005-10-4048/4/zh80080694130003.jpeg?Expires=1724233317&Signature=erOSGZgP6yEa7wcojoKzkECHyhWDWsVczJw2dgEjtV13J8Ojsypqcdxpzr5c~5J5pe2BWoXz0dn8x7rviP-uXevYKBObYqHPq-CW0UfL2dIgGvBCCPxDpQ1bglH-BYEFsfUmsB5SfJVlXuJwF9KGlmG~m2Ks9uA15PeSF1ASINn9xG9rzHxx2os~NMP51NC2tgNyUzjQN6nh06Ebi~MBqqc8aMoohwnF2eNEZLEkrKcN-NzbdeFMwA2YXKOoHenhsyGDgvBa0v8gvMGNjiQcP4ZWRh~dVetrAyOE7OxYvgQrvY229g~Vr5NkLODixAL5QrPkGkYx~8dgLLP0OdUzUg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genomic PCR-SSCP analysis of RFC exon 3 in parental cells and their PT523-resistant sublines. High-molecular-weight gDNA (∼50 ng) isolated from parental CCRF-CEM cells and their various antifolate-resistant sublines was PCR amplified in the presence of [32P]dCTP using oligonucleotide primers targeting exon 3. The PCR products were resolved by electrophoresis on nondenaturing, 8% polyacrylamide gels, as described in “Materials and methods.” The arrowhead shown on the right side depicts the band with the altered electrophoretic mobility.
