Identification of sialyl 6-sulfo Le^x as CLA and variant sialyl Le^x. (A) Results of 4-color analyses of normal peripheral blood lymphocytes using HECA-452 or 2F3 with anti-CD4, anti-CD45RO, and G152 antibodies. Two-dimensional distribution of sialyl 6-sulfo Le^x (G152) with CLA (HECA-452) or variant sialyl Le^x (2F3) on a gated CD4⁺CD45RO⁺ cell population was plotted. (B) Inhibition of staining of sialyl 6-sulfo Le^x (biotin-G152) by HECA-452 (anti-CLA), 2F3 (anti–variant sialyl Le^x), or CSLEX-1 (anti–sialyl Le^x) antibody. Results of 4-color analyses using anti-CD3, anti-CD4, anti-CD45RO, and G152 antibodies are shown. Two-dimensional distribution of sialyl 6-sulfo Le^x with CD45RO on gated CD3⁺CD4⁺ T-cell population was plotted. (C) Inhibition of staining of CLA (HECA-452) by G152 (anti–sialyl 6-sulfo Le^x) or CSLEX-1 (anti–sialyl Le^x) antibody. Results of 4-color analyses using anti-CD3, anti-CD4, anti-CD45RO, and HECA-452 antibodies. Two-dimensional distribution of CLA with CD45RO on gated CD3⁺CD4⁺ T-cell population was plotted.