Distribution of sialyl 6-sulfo Le^x and 6-sulfo Le^x determinants on human peripheral lymphocyte subsets. (A) Distribution of sialyl 6-sulfo Le^x (G152) determinants on human peripheral lymphocyte subsets. (Left column) Two-dimensional distribution of CD3, CD19, or CD56 and sialyl 6-sulfo Le^x in total lymphocytes (2-color analyses). (Middle column) Two-dimensional distribution of CD4 or CD8 and sialyl 6-sulfo Le^x in gated CD3⁺ cells (3-color analyses). (Right column) Two-dimensional distribution of CD45RO or CD45RA and sialyl 6-sulfo Le^x on gated CD3⁺CD4⁺ cells (4-color analyses). (B) Distribution of nonsialylated 6-sulfo Le^x (AG107) determinants on sialidase-treated human peripheral lymphocyte subsets in the same settings.