Effect of EBV latent gene expression on AID expression. (A) Schematic representation of generation of EREB2-5 cells from primary umbilical cord blood B cells. (B) Quantitative RT-PCR of AID mRNA levels normalized to CD19 in EREB2-5 cells cultured with or without estrogen for 3 days, followed by EBNA2 reinduction and analysis at the indicated time points. mRNA copy numbers were determined in duplicate; mean and variance of the 2 values are shown. (C) Western blot analysis of AID protein expression in cells cultured with and without estrogen. (D) LMP1 protein expression in the cells analyzed in panel B. ns indicates nonspecific band.