Assessment of CD18⁺ peripheral-blood leukocyte subsets in the 6 treated CLAD dogs with 1-year follow-up. The contribution of each subset was assessed in each of the 6 long-term treated dogs by flow cytometric analysis of CD18 expression on (A) granulocytes, (B) CD14⁺ monocytes, and (C) lymphocytes. Each subset was analyzed using a specific subset antibody, or was determined by the absence of CD14 staining and side scatter (SSC) profile (high SSC, granulocytes; low SSC, lymphocytes), along with an anti-CD18 antibody.