Analysis of TAL1, E2A, and HEB binding to human promoters. Chromatin immunoprecipitation experiments in Jurkat cells were performed with antibodies raised against TAL1, E2A, or HEB. Relative binding to each of the 71 promoters previously identified as TAL1 targets by ChIP on chip was analyzed by gene-specific quantitative PCR. Promoters marked in dark gray were enriched in the chromatin immunoprecipitates compared with those of the control genomic DNA; no enrichment was detected in the immunoprecipitates marked in light gray, while the empty boxes for RAB40B indicates that no suitable primers were identified to perform the quantitative PCR analysis.