CC-4047–triggered shift of lineage commitment of hematopoietic progenitors is not induced by CSF-G. Nonadherent bone marrow cells from HBM were cultured with 10 ng/mL CSF-M and 50 ng/mL RANKL for 21 days. DMSO 0.1% (control), or CC-4047 (100 μM) alone or in combination with anti–rh CSF-G neutralizing antibody (16 μg/mL) were added to the culture twice a week. Following 3 weeks of treatment, cells were fixed and stained with 23c6 antibody to detect mature multinucleated OCLs. Images were obtained as described for Figure 2.