Figure 3.
Maturation and function of DCs generated in the presence of imatinib. (A) DCs were generated from C57BL/6 bone marrow cells in the presence of 5 μM imatinib or DMSO as solvent control. Mature DCs were stained with αCD11c-FITC and αMHC class II–PE-Cy5 or αCD86-PE antibody. Numbers indicate the mean ± SEM of 5 independent cultures. One representative dot plot is shown. (B-C) DCs were generated from H8 bone marrow and 2 × 105 imatinib-treated or nontreated DCs were injected intravenously into naive C57BL/6 mice. Ten days later, the frequency of gp33-specific CD8+ T cells was determined by tetramer staining (B) and by intracellular IFNγ staining after in vitro restimulation with gp33 (C). Results are given as means ± SEM of 3 mice per group.
