MSCs express characteristic surface antigens and adhesion molecules. (A-B) MSCs were stained with monoclonal antibodies directed against the indicated antigens (filled areas) or isotype control antibodies (open areas). Inserted values represent percent positive cells. (C) RNA was extracted from 3 different MSC isolates and analyzed by RT-PCR for the presence of transcripts of the indicated adhesion receptors. (D) MSCs were passaged through a parallel plate flow chamber over uncoated glass slides at a calculated wall shear stress of 0.5 dynes/cm2. Controls were kept under static conditions during this time. Subsequently, MSCs were analyzed for the expression of adhesion receptors by flow cytometry. MSCs used for these experiments were from passages 5 to 9. *P < .05 (Student t test); ns indicates not significant.
