Detection of multilineage differentiation by multiplied SRCs. (A) Experimental protocol for the detection of multilineage differentiation by multiplied SRCs. (B) At 10 weeks after transplantation, the mice were killed and CD33⁺ and CD19⁺ populations within CD45⁺EGFP⁺ cells from bone marrow were sorted. The numbers in the panels show the percentage of the gated cells. Sorted CD19⁺EGFP⁺ and CD33⁺EGFP⁺ cells show more than 99% pure fractions. (C) PCR tracing of individual clones in CD19⁺EGFP⁺ and CD33⁺EGFP⁺ cells from mice that underwent transplantation with SFSD-cultured cells. Each clone was detected by amplifying a unique genomic-proviral junction sequence with primer pairs located in the genome (clone number shown in Table S1) and vector (LTR3). The numbers at the top indicate each mouse. The numbers on the left indicate each SRC clone.