Influence of Tlr7 and Tlr3 deficiency on FL-DC activation of by U1snRNP. FL-DCs generated from wild-type, Tlr3^–/–, and Tlr7^–/– mice were incubated with the indicated stimuli (10 μg/mL U1snRNP and poly-U with 12.5 μg/mL DOTAP; 20 μg/mL U1snRNP with 50 μg/mL Y12-antibody; CpG 2216 0.1 μM, poly-I:C 20 μg/mL) for 36 hours. (A) IFN-α and (B) IL-6 were measured in the supernatants by ELISA, and (C) CD86 expression was determined by flow cytometry. Mean values and standard deviations are shown (n = 3). Asterisks indicate significant differences between wild-type and Tlr7^–/– cells (P < .05; 2-tailed paired Student t test).