IFN-α and IL-6 production in response to U1snRNP/anti–Sm complexes. ICs formed with U1snRNP (20 μg/mL final concentration) and Y12-antibody or Y12-antibody alone (final concentration, 6.25-200 μg/mL) were added to FL-DCs, which had been cultured in regular medium or pretreated with IFN-β for 18 hours. After 36 hours of incubation, IFN-α and IL-6 were measured in the supernatant by ELISA. Results of 1 of 3 representative independent experiments are shown.