IFN-α and IL-6 induction by U1snRNP/DOTAP complexes in FL-DCs. FL-DCs were incubated with U1snRNP/DOTAP complexes (5, 10, and 20 μg/mL U1snRNP and 12.5 μg/mL DOTAP) or control stimuli (DOTAP/poly-U 20 μg/mL, DOTAP/poly-I:C 20 μg/mL, CpG 2216 0.5 μM) for 40 hours. Concentrations of IFN-α and IL-6 were measured in the supernatants by ELISA. Results of 1 of 3 representative independent experiments are shown.