Molecular evidence of the transfer of antileukemia T cells with BM. The 4 selected shared Vβ clonotypes shown in Figure 4 were sequenced to generate clonotype-specific probes. These probes were used to visualize the presence of the antileukemia Vβ clonotypes in BM cells and PBMCs of patient 12 and to track their transfer into PBMCs following A-BMT. (A) Sequences of TCR Vβ-D-Jβ regions of the clonotypes indicated by the arrowheads in Figure 4. Heteroduplex bands were excised from acrylamide gels and directly sequenced. Underlined are the sequences corresponding to the N-region probes used to track these clonotypes in the T-cell lines generated from PBMCs and BM cells. (B) Filter containing the carrier-heteroduplex analysis of the Vβ9 PCR products amplified from LDA derived from PBMCs and BM cells before and after BMT, hybridized with either Cβ-specific probe or Vβ9A or Vβ9B N-regions–specific probes, as indicated at the bottom of the filters. (C) Filter containing the carrier-heteroduplex analysis of Vβ13.2 PCR products amplified from LDA derived from PBMCs and BMCs before and after BMT, hybridized with either Cβ-specific probe or Vβ13.2 N-region–specific probes, as indicated at the bottom of the filters. (D) Filter containing the heteroduplex analysis of Vβ24 PCR products amplified from LDA derived from PBMCs and BM cells before and after BMT, hybridized with either Cβ-specific probe or Vβ24 N-region–specific probe as indicated at the bottom of the filters. Arrowheads indicate the heteroduplex bands formed by the TCR clonotypes of interest and the carrier. The other bands present in filters, that hybridize with the clonotype-specific probes, are due to heteroduplexes formed by the selected clonotypes and other amplified products in the sample.