The TCR repertoire of BM resident T cells is highly heterogeneous. cDNA was prepared from patient total BM cells and amplified with Vβ-specific oligonucleotides. Amplified PCR products were subjected to heteroduplex analysis as described in “PCR and carrier-heteroduplex analysis.” (A-B) The heteroduplex analysis performed on patients 7 and 8. Numbers on each line indicate the amplified TCR-Vβ family.