Decreased HSC pool size, committed and primitive progenitor activity in NKT-cell–deficient mice. (A) c-Kit⁺Lineage^–Sca-1⁺ (KLS) HSCs in WT (i) and NKTdef (ii) mice. Dot blots gated on Lin^– propidium iodide^– cells show c-Kit⁺Lin^–Sca-1⁺ cells. The frequency (iii) and absolute number (iv) of c-Kit⁺Lineage^–Sca-1⁺ per hind limb in WT and NKTdef mice are shown (n = 8). (B) Frequency (i) and absolute number (ii) of LTC-IC per hind limb in WT and NKTdef mice are shown (n = 4). (C) The frequency (i,iii) and absolute number (ii,iv) of CFU-GM and BFU-E per hind limb, respectively, in WT and NKTdef mice (n = 8) (*P < .05; **P < .001 by unpaired Student t test).