Abnormal morphology of Rac1^–/–;Rac2^–/– erythrocytes. (A) Wright-Giemsa staining of blood smears of WT, Rac1^–/–, Rac2^–/–, and Rac1^–/–;Rac2^–/– mice. Rac1^–/– and Rac2^–/– blood smears show mild poikilocytosis and anisocytosis. The Rac1^–/–;Rac2^–/– blood smear demonstrates severe poikilocytosis; arrows indicate the presence of hypochromia (1), polychromasia (2), and fragmented cells (3). Scale bar represents 10 μm. (B) RBC volume and hemoglobin concentration histograms obtained by automated complete blood count analysis of WT and Rac1^–/–;Rac2^–/– whole blood with an Advia Hematology Analyzer (images representative of 6 samples tested for each genotype). (C) Three-dimensional reconstruction of glutaraldehyde-fixed erythrocyte images. Images were obtained with a × 100 oil-immersed objective lens, numerical aperture 1.45. Arrows show Rac1^–/–;Rac2^–/– erythrocytes with thinned appearance especially at the central area (1), and a high frequency of bizarre microspherocytes with punctuate lesions (2). One unit represents 9.2 μm. (D) Increased iron deposits in the spleen of Rac1^–/–;Rac2^–/– mice compared with WT. Scale bar represents 100 μm.