The ontogeny of prostate Ag and oocyte Ag expression in B6AF1 mice. The mRNA of the ovarian MATER Ag is detected from birth by RT-PCR (A), and the MATER Ag is detected in ovarian oocytes in 1-day-old ovary by immunofluorescence with serum autoAb from d3tx mice with AOD (original magnification × 400). (B) The RNAs of prostate EAPA1 and EAPA2 are detectable in prostates at and after 10 days of age by RT-PCR. (C) The EAPA1 or EAPA2 Ag is detected in the prostate at and after 14 days of age by indirect immunofluorescence with serum autoAb from d3tx male mice with EAP (D; original magnification × 200 for top panels and × 400 for bottom panels). Panels A-B are reproduced from Alard et al,5 with permission of the Journal of Immunology (Copyright 2001. The American Association of Immunologists, Inc).
