Normal competitive repopulation ability and lineage distribution in vivo after BM transplantation ofSmad5^–/–cells. (A) Schematic figure showing the experimental layout of the primary and secondary transplantations. Competitive repopulation ability was measured as Ly5.2/Ly5.1 contribution. Shown is the short-term reconstitution in PB at 4 weeks, and long-term reconstitution at 16 weeks in BM from primary (B) and secondary (D) recipients. The distribution of myeloid cells (Mac1, Gr1), B cells (B220), and T cells (CD3) within the Ly5.2 population in BM from primary (C) and secondary (E) recipients was analyzed by FACS. Data represent mean values ± SD (n = 5 donors, 15 recipients). (F) Limiting dilution transplantation revealing similar repopulative capacities between BM cells from induced wt and MxCre;Smad5^fl/fl mice in all doses analyzed (5000, 10 000, 25 000, and 50 000 donor cells). Data represent mean values ± SD; n = 3 to 4 recipients per dose for wt and 7 to 8 recipients per dose for Smad5-deficient cells. ▦ indicates wt cells; ▪, Smad5-deficient cells.