ESE-3 down-regulation leads to an increase in IL-6 secretion. CD14⁺ peripheral-blood monocytes were isolated from buffy coat preparations with magnetic beads and transfected with 5 nM siRNA specific for ESE-3 (siRNA ESE3) or firefly luciferase GL2 (siRNA GL2). Mock transfection was carried out with the equivalent amount of water. After 48 hours of culturing the cells in RP10 medium supplemented with GM-CSF and IL-4, supernatants were collected. The data shown are representative of 4 independent experiments.