Real-time quantitative RT-PCR of differentially stimulated MDCs. CD14⁺ peripheral-blood monocytes were incubated for 6 days in RP10 medium supplemented with GM-CSF and IL-4. IL-10 was added from the first day of culture; stimulating substances were added 24 hours before harvesting of the cells. The amount of mRNA was analyzed in relation to GAPDH.