Epithelium-specific transcription factor ESE-3 is expressed in DCs. Untouched CD14⁺ peripheral-blood monocytes isolated by magnetic depletion and monocyte-derived DCs (MDCs) were analyzed for the expression of ESE-3 by RT-PCR using a gene-specific primer pair (A-B) and Western blotting of nuclear extracts using the ESE-3–specific monoclonal rat antibody 5A5.5 (C). For maturation of DCs, TNF-α, LPS, and IFN-γ were added to the cell cultures 24 hours before harvesting the cells. IL-10 was added from the first day of cell culture.