Doxycyclin-regulated FANCA expression. Western blot demonstrating repression of expression of FANCA in Dox-responsive clone D3–derived cell lines, with addition of 0 to 2000 ng/mL Dox to cells. Lanes: norm indicates endogenous level control, wild-type cell line; neg indicates clone D3 expressing empty vector as negative control; maternal indicates D3 cell line expressing FANCA maternal allele, at 0, 31, and 500 ng/mL Dox; and wt indicates D3 cell line expressing wild-type FANCA allele, no Dox. Arrow indicates FANCA in endogenous level lane.