Immunohistochemical and immunocytochemical analysis of ELMCs. (A) Freshly isolated human peripheral blood CD14⁺ monocytes, ELMCs, HUVECs, and MBECs were evaluated for uptake of DiI Ac-LDL, expression of VWF, ZO-1, and occludin by immunohistochemistry. ELMCs, HUVECs, and freshly isolated monocytes were also evaluated for binding of the endothelial-specific lectin UEA-1. (B) FACS analysis of freshly isolated monocytes, ELMCs, and HUVECs for expression of CD14, CD11b, CD31, VWF, and occludin was performed.