American Society of Hematology

Figure 5.

$Figure 5. CD44ICD localizes to the nucleus. (A) Raw 264.7 cells were transduced with pBMN-eGFP or pBMN-CD44ΔE-eGFP and treated or not with JLK2 (20 μM) or DAPT (10 μM) for 16 hours. Note that in DAPT-treated cells that express CD44ΔE-eGFP, not JLK2-treated cells, the nucleus does not fluoresce. Also, DAPT affects the shape of macrophages and induces the clustering of CD44ΔE-eGFP in the plasma membrane (bar is 7 μm). (B) Rat alveolar macrophages were transduced with the retroviral vector MigR1 encoding CD44ΔE and then subjected to subcellular fractionation. Fractions were analyzed by Western blotting using antibodies directed against myc, MFR (plasma membrane), p38 (cytosol), and Sm (nucleus).$

CD44ICD localizes to the nucleus. (A) Raw 264.7 cells were transduced with pBMN-eGFP or pBMN-CD44ΔE-eGFP and treated or not with JLK2 (20 μM) or DAPT (10 μM) for 16 hours. Note that in DAPT-treated cells that express CD44ΔE-eGFP, not JLK2-treated cells, the nucleus does not fluoresce. Also, DAPT affects the shape of macrophages and induces the clustering of CD44ΔE-eGFP in the plasma membrane (bar is 7 μm). (B) Rat alveolar macrophages were transduced with the retroviral vector MigR1 encoding CD44ΔE and then subjected to subcellular fractionation. Fractions were analyzed by Western blotting using antibodies directed against myc, MFR (plasma membrane), p38 (cytosol), and Sm (nucleus).

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