Figure 4.
Figure 4. p50 and p52 are essential for LTβR-induced expression of lymphoid tissue chemokines CCL21 and CXCL13. (A) Distorted CCL21 induction in p50 and p52 singly and doubly deficient MEFs in response to LTβR signaling. WT, Nfkb1-/-, Nfkb2-/-, and Nfkb1-/- Nfkb2-/- MEFs were stimulated for 24 hours with agonistic LTβR antibody and assessed for transcription of CCL21. (B) Distorted CXCL13 induction in p50 and p52 singly and doubly deficient MEFs in response to LTβR signaling. WT, Nfkb1-/-, Nfkb2-/-, and Nfkb1-/- Nfkb2-/- MEFs were stimulated for 24 hours with agonistic LTβR antibody and assessed for transcription of CXCL13. The RNA was isolated from the cells and converted to cDNA for analysis of CCL21 and CXCL13 transcription by real-time PCR. Error bars indicate a single standard deviation in the data of 3 biological replicates. Each biological replicate was done in 3 technical Q-PCR triplicates (though some reactions failed).

p50 and p52 are essential for LTβR-induced expression of lymphoid tissue chemokines CCL21 and CXCL13. (A) Distorted CCL21 induction in p50 and p52 singly and doubly deficient MEFs in response to LTβR signaling. WT, Nfkb1-/-, Nfkb2-/-, and Nfkb1-/-Nfkb2-/- MEFs were stimulated for 24 hours with agonistic LTβR antibody and assessed for transcription of CCL21. (B) Distorted CXCL13 induction in p50 and p52 singly and doubly deficient MEFs in response to LTβR signaling. WT, Nfkb1-/-, Nfkb2-/-, and Nfkb1-/-Nfkb2-/- MEFs were stimulated for 24 hours with agonistic LTβR antibody and assessed for transcription of CXCL13. The RNA was isolated from the cells and converted to cDNA for analysis of CCL21 and CXCL13 transcription by real-time PCR. Error bars indicate a single standard deviation in the data of 3 biological replicates. Each biological replicate was done in 3 technical Q-PCR triplicates (though some reactions failed).

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