Differential dynamic modulation of STAT1 levels in CD8 T cells during LCMV infection. CD8 T, non-CD8 T, and total splenic leukocytes were isolated from uninfected or LCMV-infected mice at the indicated days after challenge. Cell lysates were prepared, and STAT1 protein levels were detected by Western blot with β-actin measurement as a loading control. Data presented are representative of 2 independent experiments. Densitometry analyses of all tests demonstrated that the STAT1 values, normalized based on β-actin, for samples prepared on day 5 from non-CD8 T cells and total splenic leukocytes were at least 160% greater than those prepared from CD8 T cell.