In situ tetramer staining of HHV8-specific CD8 T cells in KS biopsies. CD8 cells appear in red; HHV8-specific tetramer⁺ cells appear in green; and LANA-specific cells appear in blue. Large 3-channel merged images are shown. Compared with large tetramer^– CD8 T-cell infiltrates in the vicinity of LANA⁺ tumoral cells (left panel), double-stained HHV8-specific CD8 T cells appear as isolated yellow cells (arrow) scarcely dispersed within the LANA^– tissue (middle panel). Detection of LANA⁺ cells by immunohistochemistry is shown as control (right panel). Magnification, × 63.