HOSCN induces oxidant-dependent transcriptional up-regulation of TF activity. (A) HUVECs were supplemented with either buffer (▵, control), 10 ng/mL LPS (□), or 150 μM HOSCN (▪) in M199 medium with 10% FBS, incubated at 37°C for the indicated times, and lysates assayed for TF activity. (B) HUVECs were exposed in the high serum system to either buffer (control), 150 μM HOSCN, or 10 ng/mL LPS, and in the presence or absence of 450 μM 5-thio-2-nitrobenzoic acid (TNB), and then incubated 5 hours prior to assay of TF activity. (C) HUVECs were exposed to buffer, 150 μM HOSCN, or 10 ng/mL LPS for the indicated time. Total cellular RNA was extracted and analyzed by RT-PCR using either TF-(252 bp) or 18S rRNA-(324 bp) specific primers. Gels were stained with SYBR Green I and imaged using ultraviolet transillumination.