Hierarchic cluster analysis of T-cell maturation profiles from patient with B-NHL and healthy donors. Hierarchic clustering was performed for the CD3⁺CD8⁺ (A) and the CD3⁺CD4⁺ (B) subsets. In each panel, rows represent individual lymphocyte samples from PBL from healthy donors; PBL, involved lymph nodes, or bone marrow from patients. Columns represent each of the 4 possible phenotypes obtained by analysis of CCR7 versus CD45RA by 4-color flow cytometry and coded as follows: T_N, CCR7⁺CD45RA⁺; T_CM, CCR7⁺CD45RA^–; T_EM, CCR7^–CD45RA^–; T_TD, CCR7^–CD45RA⁺. Lymphocyte samples were coded as follows: ○, PBL from healthy donors; □, PBL or LNs from patients with CLL; ▪, PBL or LNs from patients with FL; , PBL from patients with mantle-cell lymphoma [MCL]; ▴, bone marrow from patients with FL; ▵, bone marrow from patients with CLL. The percentage of positive cells for each of the 4 phenotype subsets was coded by levels of gray shading, as indicated at the bottom of the figure. The 3 major T-cell maturation clusters found in the CD8⁺ and CD4⁺ subsets were ranked from i to iii according to a progressive shift toward the most immature phenotypes.